saCas9 Null Mutant Protein ABM

saCas9 Null Mutant Protein

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. ;;The saCas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type saCas9. Such a saCas9 protein retains its ability to bind to genomic DNA through sgRNA:genomic DNA base pairing, however, the saCas9 Null Mutant does not induce cleavage. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression.;;The Cas9 nuclease from the bacteria <i>Staphylococcus aureus</i>, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

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În stoc

Număr Catalog 10021-K046

CategorieAfaceri și industrie > Știință și laboratorFurnizorGentaurDimensiune6.5 µg (50pmol) Volume: 50µLTipsingle

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saCas9 Null Mutant Protein

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saCas9 Null Mutant Protein

saCas9 Null Mutant Protein

saCas9 Null Mutant Protein

Cas9 Null Mutant Protein

saCas9 Nuclease Protein

Recombinant Saccharomyces cerevisiae Suppressor of lethality of KEX2 GAS1 double null mutant protein 1 (SKG1), partial

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Contact

Belgia (BE): +32 2 265 09 20

Franța (FR): +33 1 88 38 07 37

Regatul Unit (UK): +44 20 33 938 531

Polonia (PL): +48 58 710 33 44

Germania (DE): +49 241 936 88 188

Statele Unite (US): +1 408 780 09 08