RAF1 ELISA Kit (Human) (OKCD00315)
Description of target: Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.8 Publications
 <p>Manually curated information for which there is published experimental evidence.</p>
 
 
 <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.11"14-3-3 is phosphorylated by casein kinase I on residue 233. Phosphorylation at this site in vivo regulates Raf/14-3-3 interaction."_x005F_x005F_x000D_Dubois T., Rommel C., Howell S., Steinhussen U., Soneji Y., Morrice N., Moelling K., Aitken A._x005F_x005F_x000D_J. Biol. Chem. 272:28882-28888(1997) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH YWHAZ, FUNCTION.Ref.16"Raf-1 promotes cell survival by antagonizing apoptosis signal-regulating kinase 1 through a MEK-ERK independent mechanism."_x005F_x005F_x000D_Chen J., Fujii K., Zhang L., Roberts T., Fu H._x005F_x005F_x000D_Proc. Natl. Acad. Sci. U.S.A. 98:7783-7788(2001) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH MAP3K5/ASK1.Ref.17"Phosphorylation of the myosin-binding subunit of myosin phosphatase by Raf-1 and inhibition of phosphatase activity."_x005F_x005F_x000D_Broustas C.G., Grammatikakis N., Eto M., Dent P., Brautigan D.L., Kasid U._x005F_x005F_x000D_J. Biol. Chem. 277:3053-3059(2002) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF PPP1R12A, INTERACTION WITH PPP1R12A.Ref.23"Raf kinase activation of adenylyl cyclases: isoform-selective regulation."_x005F_x005F_x000D_Ding Q., Gros R., Gray I.D., Taussig R., Ferguson S.S., Feldman R.D._x005F_x005F_x000D_Mol. Pharmacol. 66:921-928(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF ADCY2; ADCY5 AND ADCY6, INTERACTION WITH ADCY2; ADCY5 AND ADCY6.Ref.24"Role of the kinase MST2 in suppression of apoptosis by the proto-oncogene product Raf-1."_x005F_x005F_x000D_O'Neill E., Rushworth L., Baccarini M., Kolch W._x005F_x005F_x000D_Science 306:2267-2270(2004) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH STK3/MST2, FUNCTION.Ref.27"p21-activated Kinase 1 (Pak1)-dependent phosphorylation of Raf-1 regulates its mitochondrial localization, phosphorylation of BAD, and Bcl-2 association."_x005F_x005F_x000D_Jin S., Zhuo Y., Guo W., Field J._x005F_x005F_x000D_J. Biol. Chem. 280:24698-24705(2005) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF BAD, PHOSPHORYLATION AT SER-338 AND SER-339 BY PAK1, SUBCELLULAR LOCATION, INTERACTION WITH BCL2.Ref.31"Regulation of the Raf-MEK-ERK pathway by protein phosphatase 5."_x005F_x005F_x000D_von Kriegsheim A., Pitt A., Grindlay G.J., Kolch W., Dhillon A.S._x005F_x005F_x000D_Nat. Cell Biol. 8:1011-1016(2006) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS KINASE, ENZYME REGULATION, PHOSPHORYLATION AT SER-259; SER-338; TYR-340; TYR-341 AND SER-621, DEPHOSPHORYLATION AT SER-338 BY PPP5C, MUTAGENESIS OF 338-SER-SER-339; 340-TYR-TYR-341; THR-491 AND SER-494.Ref.33"Raf 1 represses expression of the tight junction protein occludin via activation of the zinc-finger transcription factor slug."_x005F_x005F_x000D_Wang Z., Wade P., Mandell K.J., Akyildiz A., Parkos C.A., Mrsny R.J., Nusrat A._x005F_x005F_x000D_Oncogene 26:1222-1230(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.059 ng/mL
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Număr Catalog 247-OKCD00315CategorieAfaceri și industrie > Știință și laboratorFurnizorAviva Systems BiologyGentaurDimensiune96 WellsTipsingle
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