Human H3.3 K27M ORF cDNA lentivirus. Lentiviral particles, packaged with expression vector for K27M mutant of human H3.3 histone A (also known as H3.3 or H3-3A), transcript variant 1. An EF1α promoter drives expression of H3.3 K27M fused to a C-terminal V5 tag, and a GFP reporter which is separated from the ORF cDNA by a self-cleaving peptide.These lentiviral particles allow for easy transduction and high stable expression of the indicated ORF cDNA in mammalian cells. The lentiviral vectors have been optimized to provide high gene expression and reliable genome integration, allowing for easy establishment of long-term, stable cell lines with consistent read out of fluorescent (GFP, RFP) or luminescent (firefly luc) reporters. Transcription of the ORF cDNA fused with C-terminal epitope tag is controlled by either EF1a or CMV promoter, while expression of the reporter or selection marker (puromycin or blasticidin) is separated by a self-cleaving peptide to allow separate translation via the same mRNA transcript. In some constructs, the CMV or EF1a promoter drives expression of cDNA-Tag, while the GFP/RFP reporter and drug selection marker are driven by PGK promoter and separated by a self-cleaving peptide. The particles are capable of transducing a variety of difficult-to-transfect cells, including primary and/or thawed cells, making them an extremely versatile product for studying genes in mammalian systems. The accuracy of ORF cDNA cloning has been confirmed by sequencing and the successful protein expression has been validated by transient transfection.
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Preț : 595 €
În stoc
Număr Catalog 1142-LCV-0015-4SCategorieAfaceri și industrie > Știință și laboratorFurnizorLipExoGenGentaurDimensiune2x10^6Tipsingle
