This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NES1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NES1 in the samples is then determined by comparing the OD of the samples to the standard curve.
0.01EURAsk for quotation
Preț : Ask for quotation
Disponibil
Număr Catalog 855-ELK5437-48TCategorieAfaceri și industrie > Știință și laboratorFurnizorELK BiotechGentaurDimensiune48TTipsingle
