Deoxyribonucleases (DNases) are enzymes which are able to hydrolyze phosphodiester bonds of DNA molecules. They can be divided into two families, which differ in biochemical and biological properties—DNase I and DNase II families. The presence of DNases would affect many experimental results like PCR, so it is necessary to evaluate the presence of DNases.DNases are ubiquitous in both the environment and many biological materials. Many molecular biology experiments rely on the use of plastics, chemicals, and solutions that are free from detectable DNase activity, once these materials are contaminated with DNases, the experiments will be affected because of the DNases can degrade DNA. Since even only minute amounts of DNase contamination would ruin the experiment, it is necessary to evaluate the presence of DNase with a reasonable method.Published methods for detecting DNase such as Nucleic acid hydrolyzed gel electrophoresis and ultraviolet spectrophotometer are typically time consuming, not quantitative, and relatively insensitive. The other methods such as HPLC and electrochemical methods are limited to instrumentation. In contrast, the DNase Activity Assay Kit (Fluorescence) can detect DNase contamination within 30 minutes, and the kit is high sensitivity, easy to use. Moreover, the DNase and RNase Activity Assay Kit (Fluorescence) have been designed to work together seamlessly for simultaneous quantitative detection of DNases and RNases in a single sample.
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Număr Catalog 716-ASE-A002CategorieAfaceri și industrie > Știință și laboratorFurnizorACROBIOSYSTEMSGentaurDimensiune96testsTipsingle
