The PCSK9 (His) -LDLR Binding Assay Kit is an ELISA-type 96-well format assay designed for screening and profiling purposes. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding affinity in either neutral or acidic binding conditions. The assay takes advantage of the high sensitivity of detection of His-tag PCSK9 by Anti-His HRP-antibody. First, the LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with Anti-His HRP-antibody followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.Figure 1: Illustration of the ELISA-based PCSK9 (His) -LDLR Binding Assay Kit.Need us to run inhibitor screens or profile your compounds against PCSK9 (His) -LDLR? Check out ourPCSK9 Screening Services.
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Număr Catalog 421-78813-1CategorieAfaceri și industrie > Știință și laboratorFurnizorBPS BioscienceGentaurDimensiune96 ReactionsTipsingle
