The Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Chemiluminescence Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Omicron variant Spike S1 RBD (receptor binding domain) and human ACE2 (angiotensin-converting enzyme 2) . This kit comes in a convenient 96-well format, with Biotinylated-ACE2 (amino acids 18-740), purified Spike S1 RBD (Omicron Variant; B.1.1.529 BA.1) protein, Streptavidin-HRP, and assay buffers for 100 reactions. The SARS-CoV-2 Spike S1 RBD protein, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/ACE2 interaction.Figure 1. Assay Kit schematic.First, SARS-CoV-2 Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the efficacy of the binding of Spike S1 RBD (B.1.1.529 BA.1, Omicron Variant) to ACE2.
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Număr Catalog 421-78357CategorieAfaceri și industrie > Știință și laboratorFurnizorBPS BioscienceGentaurDimensiune96 rxns.Tipsingle
