| Cat # + Dimensiune | K739-100 |
|---|---|
| mărimea | 100 de teste |
| Metoda de detectare | Absorbanță (532 nm) sau fluorescență (Ex / Em 532/553 nm) |
| Reactivitatea speciilor | Toate |
| Aplicații | Kitul detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric. |
| Caracteristici și avantaje | • Procedură simplă • Rapid și convenabil • Test sensibil pentru măsurarea peroxidării lipidelor (LPA) într-o gamă largă de probe |
| Componente kit | • Tampon de liză MDA • Soluție de acid fosfotungstic • BHT (100X) • TBA • Standard MDA (4,17 M) |
| Conditii de depozitare | -20 ° C |
| Condiții de expediere | Pachet de gel |
| UTILIZARE | Numai pentru utilizare în cercetare! Nu se folosește la oameni. |
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Numele produsului
Kit de testare a peroxidării lipidelor (MDA) (colorimetric / fluorometric)
Vezi toate kiturile de peroxidare a lipidelor -
Metoda de detectare
Colorimetric / Fluorometric -
Tipul eșantionului
Urină, plasmă, extracte de cultură celulară, extracte de țesuturi -
Tipul testului
Cantitativ -
Sensibilitate
> 0,1 nmol / godeu -
Timp de testare
1h 20m -
Prezentarea produsului
Setul de testare a peroxidării lipidelor (MDA) (Colorimetric / Fluorometric) (ab118970) oferă un instrument convenabil pentru detectarea sensibilă a malondialdehidei (MDA).
În protocolul de testare a peroxidării lipidelor, MDA din probă reacționează cu acid tiobarbituric (TBA) pentru a genera un aduct MDA-TBA. Aductul MDA-TBA poate fi cuantificat cu ușurință colorimetric (OD = 532 nm) sau fluorometric (Ex / Em = 532/553 nm). Această analiză detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric.
Testul MDA este denumit și test TBARS.
Rezumatul protocolului de testare a peroxidării lipidelor:
– adăugați soluție de TBA la probe și standarde, incubați la 95 ° C timp de 60 de minute, răciți-vă în baia de gheață timp de 10 minute
– transferați în godeurile microplăcii
– analizați cu cititorul de microplăci
Pentru o sensibilitate mai mare, precipitați cu n-butanol, centrifugați, uscați și resuspendați peleta înainte de analiză.
Protocol chinezesc disponibil. A se vedea secțiunea de protocoale de mai jos.
Pentru un test alternativ MDA, fără etapele de încălzire necesare în testul TBARS, încercați testul MDA ab233471 .
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Note
Peroxidarea lipidelor se referă la degradarea oxidativă a lipidelor. În acest proces, radicalii liberi iau electroni din lipide (în general în membranele celulare), ducând la deteriorarea celulei. Cuantificarea peroxidării lipidelor este esențială pentru evaluarea stresului oxidativ. Peroxidarea lipidelor formează aldehide reactive, cum ar fi malondialdehida (MDA) și 4-hidroxinonica (4-HNE), ca bi-produse naturale. Malondialdehida (MDA) și 4-hidroxinonenala (4-HNE) sunt adesea folosite ca markeri ai peroxidării lipidelor și pentru a testa daunele oxidative / stresul oxidativ.
Produse asemanatoare
Consultați ghidul de testare a markerului oxidativ și ghidul de testare sau ghidul complet de testare a metabolismului pentru a afla mai multe teste pentru metaboliți, enzime metabolice, funcția mitocondrială și stresul oxidativ, precum și despre cum să testați funcția metabolică în celulele vii folosind cititorul de plăci.
De asemenea, consultați popularul kit de testare 4-HNE ab238538 ca un marker alternativ al peroxidării lipidelor și al stresului oxidativ.
Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit |
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| K2167-100 | ApexBio | 100 assays | EUR 703.2 |
Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit |
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| 22906 | AAT Bioquest | 200 Tests | EUR 334 |
Cell Meterâ„¢ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
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| 15991-200tests | AAT Bioquest | 200 tests | EUR 446 |
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Description: Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. |
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Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit |
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| 15991 | AAT Bioquest | 200 tests | EUR 446 |
Lipid peroxidation inhibitor 1 |
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| HY-U00319 | MedChemExpress | 1mg | EUR 1448.4 |
U-74389G (Lipid peroxidation blocker) |
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| SIH-205-100MG | Stressmarq | 100 mg | EUR 208.8 |
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Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water. |
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U-74389G (Lipid peroxidation blocker) |
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| SIH-205-500MG | Stressmarq | 500 mg | EUR 698.4 |
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Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water. |
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Myeloperoxidase (MPO) Peroxidation Activity Assay Kit |
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| K747-100 | Biovision | each | EUR 392.4 |
OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric) |
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| STA-805 | Cell Biolabs | 192 assays | EUR 692.4 |
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Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample. |
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Peroxide Quantitative Assay Kit (Lipid-Compatible) |
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| BSP067 | Bio Basic | 250Assays, 250preps | EUR 110.11 |
Dog Lipid peroxide ELISA kit |
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| E08L0263-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Lipid peroxide ELISA kit |
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| E08L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Lipid peroxide ELISA kit |
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| E08L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Lipid peroxide ELISA kit |
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| E02L0263-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Lipid peroxide ELISA kit |
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| E02L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Lipid peroxide ELISA kit |
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| E02L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Lipid peroxide ELISA kit |
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| E07L0263-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Lipid peroxide ELISA kit |
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| E07L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Lipid peroxide ELISA kit |
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| E07L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Lipid peroxide ELISA kit |
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| E06L0263-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Lipid peroxide ELISA kit |
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| E06L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Lipid peroxide ELISA kit |
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| E06L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Peroxidase Assay Kit |
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| abx298828-100Assays | Abbexa | 100 Assays | EUR 510 |
Peroxidase Assay Kit |
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| Z5030052 | Biochain | 100 assays | EUR 610 |
Mouse Lipid peroxide ELISA kit |
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| E03L0263-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Lipid peroxide ELISA kit |
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| E03L0263-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Lipid peroxide ELISA kit |
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| E03L0263-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Mouse Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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