Trusă de testare a peroxidării lipidelor (MDA)

 

Cat # + Dimensiune K739-100
mărimea 100 de teste
Metoda de detectare Absorbanță (532 nm) sau fluorescență (Ex / Em 532/553 nm)
Reactivitatea speciilor Toate
Aplicații Kitul detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric.
Caracteristici și avantaje • Procedură simplă
• Rapid și convenabil
• Test sensibil pentru măsurarea peroxidării lipidelor (LPA) într-o gamă largă de probe
Componente kit • Tampon de liză MDA
• Soluție de acid fosfotungstic
• BHT (100X)
• TBA
• Standard MDA (4,17 M)
Conditii de depozitare -20 ° C
Condiții de expediere Pachet de gel
UTILIZARE Numai pentru utilizare în cercetare! Nu se folosește la oameni.
mda-specific-lipid-peroxidation-assay-kit
  • Numele produsului

    Kit de testare a peroxidării lipidelor (MDA) (colorimetric / fluorometric)
    Vezi toate kiturile de peroxidare a lipidelor
  • Metoda de detectare

    Colorimetric / Fluorometric
  • Tipul eșantionului

    Urină, plasmă, extracte de cultură celulară, extracte de țesuturi
  • Tipul testului

    Cantitativ
  • Sensibilitate

    > 0,1 nmol / godeu
  • Timp de testare

    1h 20m
  • Prezentarea produsului

    Setul de testare a peroxidării lipidelor (MDA) (Colorimetric / Fluorometric) (ab118970) oferă un instrument convenabil pentru detectarea sensibilă a malondialdehidei (MDA).

    În protocolul de testare a peroxidării lipidelor, MDA din probă reacționează cu acid tiobarbituric (TBA) pentru a genera un aduct MDA-TBA. Aductul MDA-TBA poate fi cuantificat cu ușurință colorimetric (OD = 532 nm) sau fluorometric (Ex / Em = 532/553 nm). Această analiză detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric.

    Testul MDA este denumit și test TBARS.

    Rezumatul protocolului de testare a peroxidării lipidelor:
    – adăugați soluție de TBA la probe și standarde, incubați la 95 ° C timp de 60 de minute, răciți-vă în baia de gheață timp de 10 minute
    – transferați în godeurile microplăcii
    – analizați cu cititorul de microplăci
    Pentru o sensibilitate mai mare, precipitați cu n-butanol, centrifugați, uscați și resuspendați peleta înainte de analiză.

    Protocol chinezesc disponibil.  A se vedea secțiunea de protocoale de mai jos.

    Pentru un test alternativ MDA, fără etapele de încălzire necesare în testul TBARS, încercați  testul MDA ab233471 .

  • Note

    Peroxidarea lipidelor se referă la degradarea oxidativă a lipidelor. În acest proces, radicalii liberi iau electroni din lipide (în general în membranele celulare), ducând la deteriorarea celulei. Cuantificarea peroxidării lipidelor este esențială pentru evaluarea stresului oxidativ. Peroxidarea lipidelor formează aldehide reactive, cum ar fi malondialdehida (MDA) și 4-hidroxinonica (4-HNE), ca bi-produse naturale. Malondialdehida (MDA) și 4-hidroxinonenala (4-HNE) sunt adesea folosite ca markeri ai peroxidării lipidelor și pentru a testa daunele oxidative / stresul oxidativ.

    Produse asemanatoare

    Consultați ghidul de testare a markerului oxidativ și ghidul de testare sau ghidul complet de testare a metabolismului pentru a afla mai multe teste pentru metaboliți, enzime metabolice, funcția mitocondrială și stresul oxidativ, precum și despre cum să testați funcția metabolică în celulele vii folosind cititorul de plăci.

    De asemenea, consultați popularul kit de testare 4-HNE ab238538 ca un marker alternativ al peroxidării lipidelor și al stresului oxidativ.

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