Trusă de testare a peroxidării lipidelor (MDA)

 

Cat # + Dimensiune K739-100
mărimea 100 de teste
Metoda de detectare Absorbanță (532 nm) sau fluorescență (Ex / Em 532/553 nm)
Reactivitatea speciilor Toate
Aplicații Kitul detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric.
Caracteristici și avantaje • Procedură simplă
• Rapid și convenabil
• Test sensibil pentru măsurarea peroxidării lipidelor (LPA) într-o gamă largă de probe
Componente kit • Tampon de liză MDA
• Soluție de acid fosfotungstic
• BHT (100X)
• TBA
• Standard MDA (4,17 M)
Conditii de depozitare -20 ° C
Condiții de expediere Pachet de gel
UTILIZARE Numai pentru utilizare în cercetare! Nu se folosește la oameni.
mda-specific-lipid-peroxidation-assay-kit
  • Numele produsului

    Kit de testare a peroxidării lipidelor (MDA) (colorimetric / fluorometric)
    Vezi toate kiturile de peroxidare a lipidelor
  • Metoda de detectare

    Colorimetric / Fluorometric
  • Tipul eșantionului

    Urină, plasmă, extracte de cultură celulară, extracte de țesuturi
  • Tipul testului

    Cantitativ
  • Sensibilitate

    > 0,1 nmol / godeu
  • Timp de testare

    1h 20m
  • Prezentarea produsului

    Setul de testare a peroxidării lipidelor (MDA) (Colorimetric / Fluorometric) (ab118970) oferă un instrument convenabil pentru detectarea sensibilă a malondialdehidei (MDA).

    În protocolul de testare a peroxidării lipidelor, MDA din probă reacționează cu acid tiobarbituric (TBA) pentru a genera un aduct MDA-TBA. Aductul MDA-TBA poate fi cuantificat cu ușurință colorimetric (OD = 532 nm) sau fluorometric (Ex / Em = 532/553 nm). Această analiză detectează niveluri de MDA de la 1 nmol / godeu colorimetric și 0,1 nmol / godeu fluorometric.

    Testul MDA este denumit și test TBARS.

    Rezumatul protocolului de testare a peroxidării lipidelor:
    – adăugați soluție de TBA la probe și standarde, incubați la 95 ° C timp de 60 de minute, răciți-vă în baia de gheață timp de 10 minute
    – transferați în godeurile microplăcii
    – analizați cu cititorul de microplăci
    Pentru o sensibilitate mai mare, precipitați cu n-butanol, centrifugați, uscați și resuspendați peleta înainte de analiză.

    Protocol chinezesc disponibil.  A se vedea secțiunea de protocoale de mai jos.

    Pentru un test alternativ MDA, fără etapele de încălzire necesare în testul TBARS, încercați  testul MDA ab233471 .

  • Note

    Peroxidarea lipidelor se referă la degradarea oxidativă a lipidelor. În acest proces, radicalii liberi iau electroni din lipide (în general în membranele celulare), ducând la deteriorarea celulei. Cuantificarea peroxidării lipidelor este esențială pentru evaluarea stresului oxidativ. Peroxidarea lipidelor formează aldehide reactive, cum ar fi malondialdehida (MDA) și 4-hidroxinonica (4-HNE), ca bi-produse naturale. Malondialdehida (MDA) și 4-hidroxinonenala (4-HNE) sunt adesea folosite ca markeri ai peroxidării lipidelor și pentru a testa daunele oxidative / stresul oxidativ.

    Produse asemanatoare

    Consultați ghidul de testare a markerului oxidativ și ghidul de testare sau ghidul complet de testare a metabolismului pentru a afla mai multe teste pentru metaboliți, enzime metabolice, funcția mitocondrială și stresul oxidativ, precum și despre cum să testați funcția metabolică în celulele vii folosind cititorul de plăci.

    De asemenea, consultați popularul kit de testare 4-HNE ab238538 ca un marker alternativ al peroxidării lipidelor și al stresului oxidativ.

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

22906 200 Tests
EUR 367.2

Lipid peroxidation inhibitor 1

HY-U00319 1mg
EUR 1448.4

Cell Meter™ Intracellular Colorimetric Lipid Peroxidation (MDA) Assay Kit

15991 200 tests
EUR 471.6

U-74389G (Lipid peroxidation blocker)

SIH-205-100MG 100 mg
EUR 208.8
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

U-74389G (Lipid peroxidation blocker)

SIH-205-500MG 500 mg
EUR 698.4
Description: The substance U-74389G is a lipid peroxidation blocker. It is synthetically produced and has a purity of >99%. The pure substance is white solid which is soluble in 25 mg/ml DMSO, and in 20 mg/ml in ethanol; Insoluble in water.

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit

K747-100 each
EUR 392.4

MicroGram Lipid Assay Kit

LIP1000 1000 assays
EUR 263.3
Description: This product includes 0.1 ml of 1000 x LIP 10 Dye. It is for measurement of 1000 samples using 96-well plates. Cuvettes may also be used for measurements.

OxiSelect Myeloperoxidase Peroxidation Activity Assay Kit (Fluorometric)

STA-805 192 assays
EUR 692.4
Description: Myeloperoxidase (MPO) is a heme-based peroxidase enzyme responsible for antimicrobial activity against a wide range of organisms. It has also been found to be correlated with increased oxidative stress and decreased levels of catalase activity. The OxiSelect Myeloperoxidase Chlorination Activity Assay Kit is a quantitative, fluorometric assay for measuring the myeloperoxidase peroxidation activity within a sample.

Peroxide Quantitative Assay Kit (Lipid-Compatible)

BSP067 250Assays, 250preps
EUR 110.11

Lipid Bilayer Permeability Disruption Assay Kit

BPD100K 100 assays
EUR 325.59
Description: This product includes 1.25 ml of liposomal dye, 1.25 ml of color development solution, 7.5 ml of buffer and 1 ml of DMSO. It is for assays of 100 samples using a 96-well plate.

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

22730 200 Tests
EUR 262.8

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence*

22735 500 Tests
EUR 262.8

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

Lipid Quantitation Kit (Colorimetric)

STA-613 100 assays
EUR 637.2
Description: Cell Biolabs? Lipid Quantification Kit measures the lipid content (unsaturated fatty acids only) of samples using the sulfo-phospho-vanillin method, resulting in a simple colorimetric readout amenable to multi-well plate detection.

Lipid Quantification Kit (Fluorometric)

STA-617 100 assays
EUR 637.2
Description: The Lipid Quantification Kit (Fluorometric) uses a fluorometric detection system to quantify neutral lipids in a convenient 96-well microplate reader. The kit includes sufficient reagents for 100 assays.

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

Lipid Extraction & Polar/Neutral Lipid Separation Combo Kit (Chloroform Free)

MET-5009-C 50 preps
EUR 790.8
Description: This kit combines a chloroform free organic lipid extraction with further separation of polar and neutral lipids. The lipid extraction first extracts lipids from a crude lipid source to a chloroform free upper organic phase using a proprietary alcohol. After addition of a proprietary organic compound, the mixture is centrifuged to separate the phases. The recovered organic phase containing lipids is dried and then polar and neutral lipids are separated by adding a proprietary secondary alcohol and organic compound. The layers are separated, dried, and resuspended for further analysis.

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

Goat Lipid peroxide ELISA kit

E06L0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit

E06L0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Lipid peroxide ELISA kit

E06L0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit

E04L0263-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit

E04L0263-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Lipid peroxide ELISA kit

E04L0263-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Lipid peroxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Leave a Comment

Adresa ta de email nu va fi publicată. Câmpurile obligatorii sunt marcate cu *