INTRODUCERE
Setul de numărare a celulelor îmbunătățite Elabscience ® 8 (WST-8 / CCK8) este un kit rapid și extrem de sensibil bazat pe WST-8, care este utilizat pe scară largă în proliferarea celulelor și detectarea citotoxicității și este mai sensibil decât kitul obișnuit CCK8.

Principiul detectării
WST-8 este un compus similar cu MTT, care poate fi redus la formazan portocaliu de către o anumită dehidrogenază din mitocondrii în prezența reactivului de cuplare a electronilor. Cu cât celulele proliferează mai repede și mai repede, cu atât culoarea este mai închisă și cu cât este mai citotoxică, cu atât culoarea este mai deschisă. Pentru aceleași celule, există o relație liniară între adâncimea de culoare și numărul de celule.
Componente
Pisică. |
Produse |
100 de teste |
500 de teste |
10000 de teste |
Depozitare |
E-CK-A362 |
Buffer CCK-8 îmbunătățit |
1 ml |
5 ml |
100 ml |
-20 ° C |
Manual |
Un exemplar |
Depozitare
A se păstra la 2 ~ 8 ° C timp de un an în întuneric. Pentru depozitare îndelungată, vă rugăm să depozitați la -20 ° C, iar durata de valabilitate poate fi de doi ani.
Precauții
- Pentru depozitare îndelungată, vă rugăm să depozitați la -20 ° C. Pentru utilizare obișnuită, vă rugăm să păstrați la 2 ~ 8 ° C. Evitați ciclurile de îngheț / dezgheț.
- Amestecarea suspensiei celulare înainte de adăugarea în godeuri pentru a evita precipitarea celulei ducând la un număr diferit de celule în fiecare godeu.
- Timpul de reacție optim al CCK-8 depinde de timpul optim specific de dezvoltare a culorii. Se sugerează că numărul celulei optime și timpul de incubație al CCK-8 ar trebui explorate prin pre-experimentarea CCK-8.
- Detectarea acestui kit depinde de reacțiile catalizate de dehidrogenază, astfel încât reductanții (cum ar fi unii antioxidanți) pot interfera cu detectarea. Dacă există mai multe substanțe reductoare în sistem de detectat, încercați să le eliminați.
- Pentru siguranță și sănătate, vă rugăm să purtați haina de laborator și mănușile de unică folosință înainte de experimente.
BrdU Cell Proliferation Assay Kit |
K306-200 |
Biovision |
|
EUR 392 |
CytoSelect BrdU Cell Proliferation ELISA Kit |
CBA-251 |
Cell Biolabs |
96 assays |
EUR 531 |
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody. When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells. Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection). Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU. The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation. |
CytoSelect MTT Cell Proliferation Assay |
CBA-252 |
Cell Biolabs |
960 assays |
EUR 409 |
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. |
CytoSelect Cell Proliferation Assay Reagent
(Fluorometric) |
CBA-250 |
Cell Biolabs |
10 mL |
EUR 409 |
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then incubated with the proliferation reagent. Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. |
CytoSelect Cell Proliferation Assay Reagent (Colorimetric) |
CBA-253 |
Cell Biolabs |
10 mL |
EUR 409 |
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation. The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. Cells can be plated and then treated with compounds or agents that affect proliferation. Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. |
Cell Proliferation Assay Kit |
55R-1363 |
Fitzgerald |
500 assays |
EUR 381 |
Description: Assay Kit for detection of Cell Proliferation in the research laboratory |
Cell Proliferation Assay Kit |
55R-1364 |
Fitzgerald |
500 assays |
EUR 381 |
Description: Assay Kit for detection of Cell Proliferation in the research laboratory |
Cell Proliferation Assay Kit (Fluorometric) |
K307-1000 |
Biovision |
|
EUR 408 |
CellCount-Blue Cell Proliferation Assay Kit |
C0100-025 |
GenDepot |
250 Assays |
EUR 111 |
CellCount-Blue Cell Proliferation Assay Kit |
C0100-050 |
GenDepot |
500 Assays |
EUR 134 |
CellCount-Blue Cell Proliferation Assay Kit |
C0100-100 |
GenDepot |
1000 Assays |
EUR 188 |
CellCount-Blue Cell Proliferation Assay Kit |
C0100-500 |
GenDepot |
5000 Assays |
EUR 533 |
Cell Proliferation Assay Kit, WST-1 |
C0200-025 |
GenDepot |
250 Assays |
EUR 219 |
Cell Proliferation Assay Kit, WST-1 |
C0200-050 |
GenDepot |
500 Assays |
EUR 313 |
Cell Proliferation Assay Kit, WST-1 |
C0200-100 |
GenDepot |
1000 Assays |
EUR 469 |
MTT Cell Proliferation Assay Kit (Colorimetric) |
K299-1000 |
Biovision |
|
EUR 316 |
MTS Cell Proliferation Colorimetric Assay Kit |
K300-10000 |
Biovision |
|
EUR 1643 |
MTS Cell Proliferation Colorimetric Assay Kit |
K300-250 |
Biovision |
|
EUR 196 |
MTS Cell Proliferation Colorimetric Assay Kit |
K300-2500 |
Biovision |
|
EUR 577 |