Set îmbunătățit de numărare celule 8 (WST-8 / CCK8) [362]

INTRODUCERE

Setul de numărare a celulelor îmbunătățite Elabscience ^®  8 (WST-8 / CCK8) este un kit rapid și extrem de sensibil bazat pe WST-8, care este utilizat pe scară largă în proliferarea celulelor și detectarea citotoxicității și este mai sensibil decât kitul obișnuit CCK8.

Principiul detectării

WST-8 este un compus similar cu MTT, care poate fi redus la formazan portocaliu de către o anumită dehidrogenază din mitocondrii în prezența reactivului de cuplare a electronilor. Cu cât celulele proliferează mai repede și mai repede, cu atât culoarea este mai închisă și cu cât este mai citotoxică, cu atât culoarea este mai deschisă. Pentru aceleași celule, există o relație liniară între adâncimea de culoare și numărul de celule.

Componente

Depozitare

A se păstra la 2 ~ 8 ° C timp de un an în întuneric. Pentru depozitare îndelungată, vă rugăm să depozitați la -20 ° C, iar durata de valabilitate poate fi de doi ani.

Precauții

1. Pentru depozitare îndelungată, vă rugăm să depozitați la -20 ° C. Pentru utilizare obișnuită, vă rugăm să păstrați la 2 ~ 8 ° C. Evitați ciclurile de îngheț / dezgheț.
2. Amestecarea suspensiei celulare înainte de adăugarea în godeuri pentru a evita precipitarea celulei ducând la un număr diferit de celule în fiecare godeu.
3. Timpul de reacție optim al CCK-8 depinde de timpul optim specific de dezvoltare a culorii. Se sugerează că numărul celulei optime și timpul de incubație al CCK-8 ar trebui explorate prin pre-experimentarea CCK-8.
4. Detectarea acestui kit depinde de reacțiile catalizate de dehidrogenază, astfel încât reductanții (cum ar fi unii antioxidanți) pot interfera cu detectarea. Dacă există mai multe substanțe reductoare în sistem de detectat, încercați să le eliminați.
5. Pentru siguranță și sănătate, vă rugăm să purtați haina de laborator și mănușile de unică folosință înainte de experimente.

BrdU Cell Proliferation Assay Kit
K306-200	Biovision		EUR 392

CytoSelect BrdU Cell Proliferation ELISA Kit
CBA-251	Cell Biolabs	96 assays	EUR 531
Description: The CytoSelect BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.

CytoSelect MTT Cell Proliferation Assay
CBA-252	Cell Biolabs	960 assays	EUR 409
Description: Cell Biolabs? CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation.  The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates or 192 assays in 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1).  An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

CytoSelect Cell Proliferation Assay Reagent (Fluorometric)
CBA-250	Cell Biolabs	10 mL	EUR 409
Description: Cell Biolabs? CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then incubated with the proliferation reagent.  Upon entering metabolically active live cells, the non-fluorescent proliferation reagent is converted into a bright red fluorescent form. An increase in cell proliferation is accompanied by increased fluorescent signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.

CytoSelect Cell Proliferation Assay Reagent (Colorimetric)
CBA-253	Cell Biolabs	10 mL	EUR 409
Description: Cell Biolabs? CytoSelect WST-1 Cell Proliferation Assay Reagent provides a colorimetric format for measuring and monitoring cell proliferation.  The 10 mL volume is sufficient for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates.  Cells can be plated and then treated with compounds or agents that affect proliferation.  Cells are then detected with the proliferation reagent, which is converted in live cells from WST-1 to the formazan form in the presence of cellular NADH and an electron mediator. An increase in cell proliferation is accompanied by increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions.  The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues.  This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells.

Cell Proliferation Assay Kit
55R-1363	Fitzgerald	500 assays	EUR 381
Description: Assay Kit for detection of Cell Proliferation in the research laboratory

Cell Proliferation Assay Kit
55R-1364	Fitzgerald	500 assays	EUR 381
Description: Assay Kit for detection of Cell Proliferation in the research laboratory

Cell Proliferation Assay Kit (Fluorometric)
K307-1000	Biovision		EUR 408

CellCount-Blue Cell Proliferation Assay Kit
C0100-025	GenDepot	250 Assays	EUR 111

CellCount-Blue Cell Proliferation Assay Kit
C0100-050	GenDepot	500 Assays	EUR 134

CellCount-Blue Cell Proliferation Assay Kit
C0100-100	GenDepot	1000 Assays	EUR 188

CellCount-Blue Cell Proliferation Assay Kit
C0100-500	GenDepot	5000 Assays	EUR 533

Cell Proliferation Assay Kit, WST-1
C0200-025	GenDepot	250 Assays	EUR 219

Cell Proliferation Assay Kit, WST-1
C0200-050	GenDepot	500 Assays	EUR 313

Cell Proliferation Assay Kit, WST-1
C0200-100	GenDepot	1000 Assays	EUR 469

MTT Cell Proliferation Assay Kit (Colorimetric)
K299-1000	Biovision		EUR 316

MTS Cell Proliferation Colorimetric Assay Kit
K300-10000	Biovision		EUR 1643

MTS Cell Proliferation Colorimetric Assay Kit
K300-250	Biovision		EUR 196

MTS Cell Proliferation Colorimetric Assay Kit
K300-2500	Biovision		EUR 577