Description of target: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity.2 Publications
 <p>Manually curated information for which there is published experimental evidence.</p>
 
 
 <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"Human TOP3: a single-copy gene encoding DNA topoisomerase III."_x005F_x005F_x000D_Hanai R., Caron P.R., Wang J.C._x005F_x005F_x000D_Proc. Natl. Acad. Sci. U.S.A. 93:3653-3657(1996) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), FUNCTION, CATALYTIC ACTIVITY.Ref.11"Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1."_x005F_x005F_x000D_Yang J., Bachrati C.Z., Ou J., Hickson I.D., Brown G.W._x005F_x005F_x000D_J. Biol. Chem. 285:21426-21436(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, COFACTOR, INTERACTION WITH RMI1. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.053 ng/mL
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Număr Catalog 247-OKCD00409CategorieAfaceri și industrie > Știință și laboratorFurnizorAviva Systems BiologyGentaurDimensiune96 WellsTipsingle
