Testul de detectare a antigenului COVID-19 este un test de imunotest pentru detectarea rapidă și calitativă a infecției cu SARS-CoV-2 de la probe tamponate. Anticorpii monoclonali anti-SARS-CoV-2 sunt acoperiți pe regiunea liniei de testare. Antigenii SARS-CoV-2, dacă sunt prezenți în eșantioane, vor interacționa cu anticorpii monoclonali antiSARS-CoV-2 conjugați cu conjugatul de aur care fac complexul antigen-anticorpi conjugat. Acest complex migrează pe membrană prin acțiune capilară, unde va fi captat de anticorpul monoclonal anti-SARS-CoV-2 acoperit pe linia de testare. O linie de test colorată ar fi vizibilă în fereastra de rezultat dacă antigenii SARS-CoV-2 sunt prezenți în specimen. Intensitatea liniei de testare colorate va varia în funcție de cantitatea de antigen SARS-CoV-2 prezent în specimen.
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Îndrumări pentru utilizarea COVID-19 Ag Respi-Strip (CorisBioConcept)
ICMR a validat recent 13 truse de testare antigen COVID-19. Dintre acestea, 3 kituri au fost aprobate pentru utilizare până acum: 1. STANDARD Q COVID-19 Ag de SD Biosensor 2. COVID-19 Antigen Lateral Test Device of Lab Care Diagnostics Ltd. 3. * COVID-19 Ag Respi-Strip ( CorisBioConcept) * COVID-19 Ag Respi-Strip (Coris BioConcept) implică o metodologie diferită de testare în comparație cu celelalte două truse de testare a antigenului aprobate de ICMR până acum. Acest test nu poate fi efectuat la pat și necesită o configurare BSL-2 pentru a rula testul.
Scurtă metodologie de utilizare a COVID-19 Ag Respi-Strip (CorisBioConcept):
1. Tamponul nazofaringian și / sau orofaringian va fi colectat de la pacientul suspect COVID-19 în mediu de transport viral (VTM). 2. Tamponul colectat în VTM va fi adus la laborator în condiții corespunzătoare ale lanțului de frig. 3. Odată ce proba este adusă la laborator, va trebui manipulată într-un dulap de nivel BSL-2 pentru alicotare, punerea în tampon de liză și încărcarea benzii de testare. Etapele 1 și 2 vor fi efectuate conform practicii standard urmate pentru colectarea și transportul probelor pentru testul COVID-19 RT-PCR. Pasul 3 va trebui efectuat conform instrucțiunilor producătorilor furnizate împreună cu kitul de testare.
Description: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection (3). The spike protein is the major target for neutralizing antibodies and vaccine development (4). The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19 (5). The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein (6).
Description: These antibodies bind to two spike proteins (S1 & S2) on the SARS-CoV-2 virus and can detect presence of SARS-CoV-2 ELISA test COVID-19 spike protein using antibody pair ab-131-295-PAIR. Antibodies: Rabbit anti-spike protein S1 was coated at a dilution of 1/1000 in PBS for 1 hour at room temperature. After blocking, calibrator was diluted from 2000ng/ml to 15ng/m1 in blocking buffer and incubated for 1 hour on coated plate. After washing detection antibody (Chicken anti-spike protein S2 – HRP) was diluted 1/1000 in blocking buffer and incubated on the calibrator wells for 1 hour. After a final wash and tamping, the ELISA was developed by the addition of 100u1TMB to each well and stopped after 15 minutes by the addition of 0.2M sulfuric acid.
Description: Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.
Description: DescriptionFor research use only. Not to be used for diagnostic purposes.Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains Reverse Transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/ probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product Includes:RT-qPCR 2x master mixCDC recommended primer/probe setsCOVID-19 positive control (PTC)
One Step RT-qPCR kit for SARSCoV-2 (COVID-19) detections - 500 Reactions
Description: DescriptionFor research use only. Not to be used for diagnostic purposes.Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of cases of pneumonia outbreak. This Coronavirus detection kit allows efficient cDNA synthesis and qPCR in a single tube. This probe based one step RTqPCR 2x master mix contains Reverse Transcriptase, Taq DNA polymerase, RNase inhibitor, MgCl2, dNTPs, stabilizers and low ROX reference dye with proprietary buffer providing improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/ probe sets. This kit can be used to detect SARS-CoV-2 in respiratory specimens such as sputum, nasopharyngeal, oropharyngeal aspirates, washes or swabs and tracheal aspirates.IgScript™ Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity, increased thermostability and can produce cDNA from small amount of total RNA for real-time RT-qPCR analysis and other applications. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.Product Includes:RT-qPCR 2x master mixCDC recommended primer/probe setsCOVID-19 positive control (PTC)
