Imobilizarea dirijată la fața locului a anticorpului

utilizând proteina A activată EDC-NHS pe un cip de rezonanță plasmonică pe bază de suprafață bimetalică

Abstract

Caracteristicile unui senzor de rezonanță plasmonică de suprafață bimetalică cuplat cu ghid de undă (WcBiM SPR) utilizând proteina A (3-dimetilaminopropil) -3-etilcarbodiimidă (EDC) -N-hidroxisuccinimidă (NHS) a fost investigată, iar detectarea IgG utilizând Proteina A activată prin EDC-NHS a fost studiată în comparație cu proteina A și un monostrat auto-asamblat (SAM). Senzorul WcBiM, care are o lățime completă mai îngustă la jumătate maximă (FWHM) și o pantă mai abruptă, a fost selectat deoarece duce la o schimbare mai mare a reflectanței în modul de detectare a intensității. Un preparat al proteinei A activate prin EDC-NHS pentru imobilizarea direcționată la fața locului a anticorpilor a fost relativ ușor în comparație cu proteinele G și A. modificate. În interacțiunile antigen-anticorp, răspunsul la IgG la concentrațiile de 50, 100 și 150 ng / ml a fost investigat. Rezultatele au arătat că sensibilitatea senzorului WcBiM utilizând proteina A, proteina A și SAM activată EDC-NHS a fost de 0,0185 [% / (ng / ml)], 0,0065 [% / (ng / ml)] și 0,0101 [% / (ng / ml)], respectiv.

Cele mai scăzute concentrații detectabile de IgG cu proteina A activată EDC-NHS, proteina A și SAM au fost de 4,27, 12,83 și respectiv 8,24 ng / ml. Prin urmare, sensibilitatea crescută și capacitatea mai mică de detectare a cipului WcBiM SPR cu proteina A activată EDC-NHS sugerează că ar putea fi utilizată în diagnosticul precoce în care ar trebui detectate concentrațiile de urme ale biomoleculelor. proteina A și SAM au fost de 4,27, 12,83 și respectiv 8,24 ng / ml. Prin urmare, sensibilitatea crescută și capacitatea mai mică de detectare a cipului WcBiM SPR cu proteina A activată EDC-NHS sugerează că ar putea fi utilizată în diagnosticul precoce în care ar trebui detectate concentrațiile de urme ale biomoleculelor. proteina A și SAM au fost de 4,27, 12,83 și respectiv 8,24 ng / ml. Prin urmare, sensibilitatea crescută și capacitatea mai mică de detectare a cipului WcBiM SPR cu proteina A activată EDC-NHS sugerează că ar putea fi utilizată în diagnosticul precoce în care ar trebui detectate concentrațiile de urme ale biomoleculelor.

Detalii produs

Numele complet al produsului Iepure anti-Escherichia coli (tulpina K12) hns Anticorp policlonal
Uniprot Nr. P0ACF8
Numele țintei hns
Denumiri alternative anticorp hns; anticorp bglY; cur anticorp; anticorp drdX; anticorp hnsA; msyA anticorp; anticorp osmZ; anticorp pilG; anticorp topS; anticorp b1237; Anticorp JW1225; Anticorp H-NS de proteină de legare a ADN-ului; Anticorp proteic structurant nucleoid stabil de căldură; Anticorp proteic HLP-II asemănător histonelor; Anticorp proteic B1; Anticorp proteic H1
Crescut în Iepure
Reactivitatea speciilor Escherichia coli
Imunogen Proteina recombinantă Escherichia coli care leagă ADN proteina H-NS (39-137AA)
Specii imunogene Escherichia coli (tulpina K12)
Conjuga Neconjugat
Clonalitate Policlonal
Isotip IgG
Metoda de purificare > 95%, proteina G purificată
Concentraţie Diferă de diferite loturi. Vă rugăm să ne contactați pentru confirmare.
Tampon Conservant: 0,03% Proclin 300
Constituenți: 50% glicerol, 0,01 M PBS, pH 7,4
Formă Lichid
Aplicații testate ELISA, BM
Diluarea recomandată
Cerere Diluarea recomandată
WB 1: 2000-1: 10000
Protocoale Protocolul ELISA Protocolul
Western Blotting (WB)
Depanare și întrebări frecvente Întrebări frecvente despre anticorpi
Depozitare La primire, depozitați la -20 ° C sau -80 ° C. Evitați înghețarea repetată.
Perioada de grație Practic, putem expedia produsele în 1-3 zile lucrătoare de la primirea comenzilor dvs. Timpul de livrare poate diferi de modul sau locația de cumpărare diferită, vă rugăm să consultați distribuitorii locali pentru un timp de livrare specific.

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